Journal: Cancer Research

Article Title: Nerves Stimulate Cross-talk Between Gastric Cancer and Group 3 Innate Lymphoid Cells to Enhance Immunosuppression

doi: 10.1158/0008-5472.CAN-25-3092

Figure Lengend Snippet: Elevated ABHD16A triggered by ACh was associated with poor prognosis in patients with gastric cancer. A, Representative IHC images of ABHD16A expression in human normal gastric tissues and gastric cancer tissues from the TMA with magnifications of 40×, 100×, and 200×. Scale bar for 200×, 100 μm. B–D, Relationship of ABHD16A IHC score to clinical stage ( B ), pT stage ( C ), and lymph node metastasis ( n = 80; D ). E, Cumulative survival curves of patients with gastric cancer with high or low ABHD16A expression based on the TMA. F, IHC images and score of ABHD16A in S100 + or S100 − gastric cancer tissues. Scale bar, 100 μm. G, Procedure for coculture of DRG neurons and gastric cancer cells. H, Western blotting was used to analyze ABHD16A levels in MFC cells cocultured with DRG neurons or DRG neuron–derived CM. GAPDH served as the loading control. I, Expression of HIF1A and ABHD16A in neurotransmitter [HA, dopamine (DA), 5-HT, norepinephrine (NE), ACh]-treated MFC cells and ACh-treated MGC-803 cells. J, ACh concentration was detected in DRG CM by ELISA. K, Tumor volume of orthotopic gastric cancer tumors with or without vagotomy. L, Representative mIF staining images of FOXP3, CD163, CD11b, CD8, PD-L1, and Pan-CK in gastric cancer tissues with high or low expression of ABHD16A. Scale bar, 100 μm. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

Article Snippet: ACh in the dorsal root ganglion (DRG)–derived conditioned medium (CM) was detected using the ACh ELISA kit (Elabscience, E-EL-0081).

Techniques: Expressing, Western Blot, Derivative Assay, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay, Staining

Journal: Translational Neuroscience

Article Title: The role of melatonin in affecting cognitive dysfunction in acute sleep deprivation mice through the nuclear factor kappaB pathway and oxidative stress

doi: 10.1515/tnsci-2025-0379

Figure Lengend Snippet: Melatonin improved CD in male C57BL/6J ASD mice. MWM test was conducted to detect (a) escape latency, (b) time needed for first crossing, (c) number of crossings through the target platform zone, and (d) time spent in the target platform quadrant within 180 s of mice ( n = 12); (e) open field experiment was performed to assess mouse spontaneous activity ( n = 12); (f) elevated plus maze test was conducted to assess time spent in open and enclosed arms ( n = 12); (g) ELISA was used to measure AChE activity and ACh levels in the hippocampal CA1 region of SD mice ( n = 6). Data in panel (c) failed the Shapiro–Wilk test for normality and the Mann–Whitney U -test was applied for inter-group comparisons. All other data were normally distributed, with the results presented as mean ± standard deviation. (a) Escape latency in the MWM test was analyzed using two-way ANOVA, with the Šídák’s multiple comparison test used for post hoc analyses; (b)–(g) one-way ANOVA was applied for comparisons among multiple groups, and Tukey’s multiple comparison test for post hoc analysis. * P < 0.05, ** P < 0.01.

Article Snippet: ELISA kits were used to determine levels of IL-4 (Mouse IL-4 ELISA Kit, ml063156, Enzyme-linked Biotechnology, Shanghai, China), IL-10 (Mouse IL-10 ELISA Kit, ml037873, Enzyme-linked Biotechnology), TNF-α (Mouse TNF-α ELISA Kit, ml002095, Enzyme-linked Biotechnology), IL-1β (Mouse IL-1β ELISA Kit, ml301814, Enzyme-linked Biotechnology), acetylcholine (ACh) (mouse ACh ELISA kit, E-EL-0081, Elabscience, Wuhan, Hubei.

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, Standard Deviation, Comparison

Journal: Translational Neuroscience

Article Title: The role of melatonin in affecting cognitive dysfunction in acute sleep deprivation mice through the nuclear factor kappaB pathway and oxidative stress

doi: 10.1515/tnsci-2025-0379

Figure Lengend Snippet: Melatonin mitigated neuroinflammation and oxidative stress in the hippocampal CA1 region of male C57BL/6J ASD mice. (a) HE staining to observe the structure of the hippocampal CA1 region of mice; (b) Nissl staining to observe the neurons of the hippocampal CA1 region of mice; ELISA to determine TNF-α, IL-1β, IL-4, and IL-10, (c) MDA and ROS levels, and SOD activity in the hippocampal CA1 region of mice (d); (e) western blot to measure PSD95 protein expression in the hippocampal CA1 region of mice; n = 6. All data were normally distributed as determined by the Shapiro–Wilk test and were presented as mean ± standard deviation. One-way ANOVA was applied for comparisons among multiple groups, and Tukey’s multiple comparison for post hoc analysis. Multiple comparisons were subjected to FDR correction using the Benjamini–Hochberg method ( q = 0.05). Corrected P -values were denoted as P adj , while uncorrected raw P -values were labeled as P . FDR correction ( q = 0.05) was applied to all multiple comparisons within each analytical category (inflammatory cytokines, oxidative stress markers). * P < 0.05, ** P < 0.01.

Article Snippet: ELISA kits were used to determine levels of IL-4 (Mouse IL-4 ELISA Kit, ml063156, Enzyme-linked Biotechnology, Shanghai, China), IL-10 (Mouse IL-10 ELISA Kit, ml037873, Enzyme-linked Biotechnology), TNF-α (Mouse TNF-α ELISA Kit, ml002095, Enzyme-linked Biotechnology), IL-1β (Mouse IL-1β ELISA Kit, ml301814, Enzyme-linked Biotechnology), acetylcholine (ACh) (mouse ACh ELISA kit, E-EL-0081, Elabscience, Wuhan, Hubei.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Activity Assay, Western Blot, Expressing, Standard Deviation, Comparison, Labeling

Journal: Translational Neuroscience

Article Title: The role of melatonin in affecting cognitive dysfunction in acute sleep deprivation mice through the nuclear factor kappaB pathway and oxidative stress

doi: 10.1515/tnsci-2025-0379

Figure Lengend Snippet: Suppression of the NF-κB pathway improved neuroinflammation and oxidative stress in the hippocampal CA1 region of male C57BL/6J ASD mice. (a/f) Western blot to determine p-p65/p65 ratio and PSD95 protein expression in the hippocampal CA1 region of mice; (b) HE staining to observe the structure of the hippocampal CA1 region of mice; (c) Nissl staining to observe the neuronal status of the hippocampal CA1 region of mice; ELISA to determine (d) TNF-α, IL-1β, IL-4, and IL-10 levels and (e) MDA levels, ROS, and SOD activity in the hippocampal CA1 region; n = 6. Data were expressed as mean ± standard deviation. The t -test was adopted for inter-group comparisons. Multiple comparisons were subjected to FDR correction using the Benjamini–Hochberg method ( q = 0.05). Corrected P -values were denoted as P adj , while uncorrected raw P -values were labeled as P . FDR correction ( q = 0.05) was applied to all multiple comparisons within each analytical category (inflammatory cytokines, oxidative stress markers). * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: ELISA kits were used to determine levels of IL-4 (Mouse IL-4 ELISA Kit, ml063156, Enzyme-linked Biotechnology, Shanghai, China), IL-10 (Mouse IL-10 ELISA Kit, ml037873, Enzyme-linked Biotechnology), TNF-α (Mouse TNF-α ELISA Kit, ml002095, Enzyme-linked Biotechnology), IL-1β (Mouse IL-1β ELISA Kit, ml301814, Enzyme-linked Biotechnology), acetylcholine (ACh) (mouse ACh ELISA kit, E-EL-0081, Elabscience, Wuhan, Hubei.

Techniques: Western Blot, Expressing, Staining, Enzyme-linked Immunosorbent Assay, Activity Assay, Standard Deviation, Labeling

Journal: Translational Neuroscience

Article Title: The role of melatonin in affecting cognitive dysfunction in acute sleep deprivation mice through the nuclear factor kappaB pathway and oxidative stress

doi: 10.1515/tnsci-2025-0379

Figure Lengend Snippet: Inhibition of the NF-κB pathway notably attenuated CD in male C57BL/6J ASD mice. MWM test to determine (a) escape latency, (b) first crossing time, (c) number of crossings through the target platform zone, and (d) time spent in the target platform quadrant within 180 s of mice ( n = 12); (e) open field experiment to assess spontaneous activity of mice ( n = 12); (f) elevated plus maze test was conducted to assess time spent in open and enclosed arms ( n = 12); (g) ELISA was used to measure AChE activity and ACh levels in the hippocampal CA1 region of SD mice ( n = 6). Data were expressed as mean ± standard deviation. (a) Escape latency in the MWM test was analyzed using two-way ANOVA, with the Šídák’s multiple comparison test used for post hoc analyses; (b)–(g) the t test was utilized for inter-group comparisons. ** P < 0.01.

Article Snippet: ELISA kits were used to determine levels of IL-4 (Mouse IL-4 ELISA Kit, ml063156, Enzyme-linked Biotechnology, Shanghai, China), IL-10 (Mouse IL-10 ELISA Kit, ml037873, Enzyme-linked Biotechnology), TNF-α (Mouse TNF-α ELISA Kit, ml002095, Enzyme-linked Biotechnology), IL-1β (Mouse IL-1β ELISA Kit, ml301814, Enzyme-linked Biotechnology), acetylcholine (ACh) (mouse ACh ELISA kit, E-EL-0081, Elabscience, Wuhan, Hubei.

Techniques: Inhibition, Activity Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation, Comparison

Journal: Translational Neuroscience

Article Title: The role of melatonin in affecting cognitive dysfunction in acute sleep deprivation mice through the nuclear factor kappaB pathway and oxidative stress

doi: 10.1515/tnsci-2025-0379

Figure Lengend Snippet: Activation of the NF-κB signaling promoted oxidative stress and CD in the hippocampal CA1 region of male C57BL/6J ASD mice. (a/f) Western blot for determination of p-p65, p65, and PSD95 protein expression levels in the hippocampal CA1 region ( n = 6); (b) HE staining for observation of the structure of the mouse hippocampal CA1 region ( n = 6); (c) Nissl staining for assessment of neuronal status in the mouse hippocampal CA1 region ( n = 6); (d) ELISA for measurement of levels of TNF-α, IL-1β, IL-4, and IL-10 levels in the hippocampal CA1 region of mice ( n = 6); (e) levels of MDA, ROS, and SOD activity in the mouse hippocampal CA1 region ( n = 6); (f) western blot for determination of PSD95 protein expression; MWM test to detect (g) escape latency, (h) time needed for first crossing, (i) number of crossings through the target platform zone within 180 s, and (j) time spent in the target platform quadrant within 180 s of mice ( n = 12); (k) open field experiment to assess mouse spontaneous activity ( n = 12); (l) elevated plus maze test to quantify time spent in open and enclosed arms ( n = 12); (m) ELISA was used to measure AChE activity and ACh levels in the hippocampal CA1 region of SD mice ( n = 6). Data were presented as mean ± standard deviation. (g) Escape latency was analyzed using two-way ANOVA, with the Šídák’s multiple comparison test used for post hoc analyses; other panels: inter-group comparisons were analyzed by the t -test. Multiple comparisons underwent FDR correction via the Benjamini–Hochberg method ( q = 0.05). Corrected P -values were denoted as P adj and uncorrected raw P -values as P . FDR correction ( q = 0.05) was applied to all multiple comparisons within each analytical category (inflammatory cytokines, oxidative stress markers). * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: ELISA kits were used to determine levels of IL-4 (Mouse IL-4 ELISA Kit, ml063156, Enzyme-linked Biotechnology, Shanghai, China), IL-10 (Mouse IL-10 ELISA Kit, ml037873, Enzyme-linked Biotechnology), TNF-α (Mouse TNF-α ELISA Kit, ml002095, Enzyme-linked Biotechnology), IL-1β (Mouse IL-1β ELISA Kit, ml301814, Enzyme-linked Biotechnology), acetylcholine (ACh) (mouse ACh ELISA kit, E-EL-0081, Elabscience, Wuhan, Hubei.

Techniques: Activation Assay, Western Blot, Expressing, Staining, Enzyme-linked Immunosorbent Assay, Activity Assay, Standard Deviation, Comparison